Dual Labelled Probes…

We offer a broad spectrum of Probes for Real-Time PCR (TaqMan, Molecular Beacons, etc.) available in our premium grade DNA Reverse Phase HPLC.

Specification for DNA Reverse Phase HPLC (RP-HPLC) Dual Labelled Probes:

  • Deprotected & purified
  • Recommended for methods like PCR, sequencing and hybridization
  • Delivered as solid
  • Modification allowed
  • Certificate of Analysis (CoA) follows
  • 100% Quality Controlled (QC) by MassSpec
  • To be kept frozen at -18 C
  • Average in-house turnover time: 3-5 working days
  • Scale & yield
    Product Synthesis scale (µmol) Length OD Est. no. of PCR reactions
    DNA Reverse Phase HPLC 0.01 n/a n/a n/a
    DNA Reverse Phase HPLC 0.04 8-80 2 200
    DNA Reverse Phase HPLC 0.20 8-80 4 400
    DNA Reverse Phase HPLC extra long 0.20 81-120 2 200
    DNA Reverse Phase HPLC 1.00 8-80 20 20.000

List of available Dual Labelled Probes:

Price list for Probes for Real-Time PCR Probes:

How Dual Labeled Probes Work

A Dual-Labeled Probe is a single-stranded oligonucleotide labeled with two different dyes. A reporter dye is located at the 5’-end and a quencher molecule located at the 3′-end. The quencher molecule inhibits the natural fluorescence emission of the reporter dye. The primer is elongated by the polymerase and the probe binds to the specific DNA template, hydrolysis releases the reporter dye from the probe/target hybrid, causing an increase of fluorescence. The measured fluorescence signal is directly proportional to the amount of target DNA.

Please inquire should you have any other requirements.

>>> Order Dual Labelled Probes here