PCR & qPCR Kit Specifications…

TAG Copenhagen is the Danish representative for Solis BioDyne – a leading life science reagent supplier in Europe. The main focus of Solis Biodyne is the development of PCR & qPCR Reagents/Kits. Solis Biodyne offers the following PCR & qPCR products:

  • qPCR Mixes
  • PCR Mixes
  • Taq DNA Polymerases
  • Next Generation Sequencing Kits
  • Other PCR Reagents
  • Other Enzymes

A description of the PCR & qPCR Kits of Solis Biodyne is found below:

qPCR

Optimized ready-to-use solutions for real-time quantitative PCR assays with all the components necessary to perform qPCR.

  • Has mixes for both – probe based approach and intercalating dye approach (based on EvaGreen dye). Please find suitable mix for your qPCR platform from the table or product list below.

Mix compatibility table:

qPCR platform Probe based Intercalating dye based
Applied Biosystems: 7900HT, 7500, 7700, 7000, StepOne™ & StepOnePlus™
Stratagene: Mx3000P™ & Mx3005P™
5x HOT FIREPol® Probe qPCR Mix Plus (ROX) 5x HOT FIREPol® EvaGreen® qPCR Mix Plus (ROX)
Bio-Rad: CFX96™ & CFX384™, iQ™5 & MyiQ™,Chromo4™, Opticon® 2 & MiniOpticon®
Qiagen: Rotor-Gene® Q, Rotor-Gene® 6000
Eppendorf: Mastercycler®: ep realplex2 & ep
realplex4
Illumina: The Eco™
Roche: LightCycler® 480
5x HOT FIREPol® Probe qPCR Mix Plus (no ROX) 5x HOT FIREPol® EvaGreen® qPCR Mix Plus (no ROX)
Roche: LightCycler® 1.x, LightCycler® 2.0 5x HOT FIREPol® Probe qPCR Mix Plus (Capillary) 5x HOT FIREPol® EvaGreen® qPCR Mix Plus (Capillary)

FIREPol is a trademark of Solis BioDyne. Applied Biosystems and ROX are trademarks of the Applera Corporation. Stratagene, MX3005, MX3005P are trademarks of Stratagene Corporation. Mastercycler is a trademark of Eppendorf AG. Rotor-Gene is a registered trademark of Corbett Research (Qiagen). CFX96, CFX384, iQ, MyiQ, Chromo4, Opticon 2, MiniOpticon are trademarks of Bio-Rad Laboratories. LightCycler is a registered trademark of Roche Applied Sciences. The Eco is registered trademark of Illumina Inc. EvaGreen is a registered trademark of BIOTIUM, INC.

Product Data sheet MSDS  
HOT FIREPol® DNA Polymerase  Data Sheet MSDS  
5x HOT FIREPol® Blend Master Mix Ready to Load with BSA and 7.5 mM MgCl2  Data Sheet MSDS  
5x HOT FIREPol® Blend Master Mix Ready to Load with BSA and 10 mM MgCl2  Data Sheet MSDS  
5x HOT FIREPol® Blend Master Mix Ready to Load with BSA and 12.5 mM MgCl2  Data Sheet MSDS  
5x HOT FIREPol® Blend Master Mix Ready to Load with BSA and 15 mM MgCl2  Data Sheet MSDS

Hot Start PCR

  • Hot Start PCR prevents misextension from non-specifically annealed primers and primer-dimers.
  • HOT FIREPol®  DNA Polymerase is a chemically modified FIREPol®  DNA Polymerase. At ambient temperature it is inactive having no polymerization activity. HOT FIREPol® DNA Polymerase is activated by a 12-15 min incubation step at 95º C.
  • Hot-start master mixes are also available in ready-to-load format with variety of MgCl2 concentrations.
  • 5x HOT FIREPol® Blend Master Mix is a highly user-friendly and versatile tool for your Hot Start PCR. The mix is based on a blend of our HOT FIREPol®polymerase and a proofreading polymerase, providing a low error rate and high sensitivity. In addition, the mix also allows amplification of longer fragments (up to 5 kb). Here some great advantages of this ready-to-load mix:
    • decreased set-up time
    • reduced risk of contamination
    • less pipetting mistakes
    • consistent reaction to reaction performance
    • contains all reagents required for PCR
  • Like all products of Solis BioDyne, the 5x HOT FIREPol® Blend Master Mix is stable at ambient temperature for up to one month. This makes handling and set-up even more convenient, since no ice has to be used.
Product Data sheet MSDS  
HOT FIREPol® DNA Polymerase  Data Sheet MSDS  
5x HOT FIREPol® Blend Master Mix Ready to Load with BSA and 7.5 mM MgCl2  Data Sheet MSDS  
5x HOT FIREPol® Blend Master Mix Ready to Load with BSA and 10 mM MgCl2  Data Sheet MSDS  
5x HOT FIREPol® Blend Master Mix Ready to Load with BSA and 12.5 mM MgCl2  Data Sheet MSDS  
5x HOT FIREPol® Blend Master Mix Ready to Load with BSA and 15 mM MgCl2  Data Sheet MSDS  

PCR

  • The polymerase chain reaction (PCR) is a technique to amplify a short sequence of DNA even in samples containing only minute quantities of DNA. PCR is used to amplify selected sections of DNA or RNA across several orders of magnitude.
  • FIREPol® is highly processive, thermostable and robust Taq polymerase with amplification range up to 3 kb. Due to Solis Tag technology, FIREPol® has an enhanced stability at ambient temperature with no activity loss up to 1 month.
  • We supply FIREPol® Polymerase as enzyme kit with a selection of buffers and an enhancer for personal optimization.
  • Master Mixes with or without ready-to-load feature are also available.
Product Data sheet MSDS  
FIREPol® DNA Polymerase Data Sheet MSDS  
5x FIREPol® Master Mix (7.5 mM MgCl2) Data Sheet MSDS  
5x FIREPol® Master Mix (12.5 mM MgCl2) Data Sheet MSDS  
5x FIREPol® Master Mix Ready to Load (7.5 mM MgCl2) Data Sheet MSDS  
5x FIREPol® Master Mix Ready to Load (12.5 mM MgCl2) Data Sheet MSDS  

High Resolution Melt Analysis (HRM)

  • High Resolution Melting (HRM) is a hugely powerful, close-tube, post-qPCR genotyping method for the detection of mutations, polymorphisms and epigenetic differences in double stranded DNA samples.
  • It goes beyond the power of classical melting curve analysis (MCA) by allowing to study the thermal denaturation of a double-stranded DNA in much more detail. Samples can be discriminated according to their sequence, length, GC content or strand complementarity. Even single base changes such as SNPs (single nucleotide polymorphisms) can be readily identified.
  • HOT FIREPol® EvaGreen® HRM Mix is an optimized ready-to-use solution for High Resolution Melt (HRM) Analysis, incorporating EvaGreen® dye.
  • It includes:  HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2 and ROX dye according to system requirements.
Product Data sheet MSDS  
 5x HOT FIREPol® EvaGreen® HRM Mix (no ROX) Data Sheet MSDS  
 5x HOT FIREPol® EvaGreen® HRM Mix (ROX) Data Sheet MSDS  

MALDI TOF

  • MALDI-TOF (matrix-assisted laser desorption ionization time-of flight) is a genotyping technique for mass spectrometry allowing the analysis down to single base changes such as SNPs (single nucleotide polymorphisms).
  • TERMIPol® DNA Polymerase is a thermostable DNA Polymerase suited for MALDI TOF mass spectrometry and other primer extension platforms. The enzyme has enhanced efficiency for incorporating unconventional nucleotides.
Product Data sheet MSDS  
 TERMIPol® DNA Polymerase Data Sheet MSDS  
 HOT TERMIPol® DNA Polymerase Data Sheet MSDS  

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