Polyclonal Antibody Services…

Polyclonal antibodies are made from several different immune cells. Polyclonal antibodies can be used as both primary and secondary antibodies in Westerns, ELISAs and IHCs. TAG Copenhagen offers a variety of polyclonal antibody production packages. Please contact our customer services should you need any assistance for our polyclonal antibody services.

Polyclonal Antibody Services
Package Number
Description
Package Contents
Time
Polyclonal package 1
(From protein to antiserum)
l  Customer provides antigen protein
l  Protein will be checked by SDS-PAGE
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  1-2ml pre-bleed
l  40-60ml post-bleed
l  Project datasheet including ELISA data
10-12 weeks
Polyclonal package 2
(From protein to antigen purified antibody)
l  Customer provides antigen protein
l  Protein will be checked by SDS-PAGE
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  Antigen affinity purification
l  Dot blot confirmation
l  1-2ml pre-bleed
l  2-5mg affinity purified antibody
l  Project datasheet including ELISA and dot blot data
10-12 weeks
Polyclonal package 3
(From peptide design to antiserum)
l  Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
l  Synthesize one peptide (12-22aa, purity>85%)
l  Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  2-3mg antigen peptide
l  1-2ml pre-bleed
l  40-60ml post-bleed
l  Project datasheet including ELISA data
12-15 weeks
Polyclonal package 4
(From peptide design  to antigen purified antibodies)
l  Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
l  Synthesize one peptide (12-22aa, purity>85%)
l  Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  Antigen affinity purification
l  Dot blot confirmation
l  2-3mg antigen peptide
l  1-2ml pre-bleed
l  2-5mg affinity purified antibody
l  Project datasheet including ELISA and dot blot data
12-15 weeks
Polyclonal package 5 (From plasmid  to antiserum)
l  Customer provides expression vector
l  Express and purify the antigen protein
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  1mg antigen protein
l  1-2ml pre-bleed
l  40-60ml post-bleed
l  Project datasheet including ELISA data
12-15 weeks
Polyclonal package 6
(From plasmid  to antigen purified antibody)
l  Customer provides expression vector
l  Express and purify the antigen protein
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  Antigen affinity purification
l  Dot blot confirmation
l  1mg antigen protein
l  1-2ml pre-bleed
l  2-5mg affinity purified antibody
l  Project datasheet including ELISA and dot blot data
12-15 weeks
Polyclonal package 7
(From cDNA  to antiserum)
l  Customer provides protein information
l  We clone the gene and construct the expression vector for fusion protein
l  Express and purify the antigen protein
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  Recombinant vector
l  1mg protein
l  1-2ml pre-bleed
l  40-60ml post-bleed
l  Project datasheet including ELISA data
14-16 weeks
Polyclonal package 8
(From cDNA to antigen purified antibody)
l  Customer provides gene and protein information
l  We clone the gene and construct the expression vector for fusion protein
l  Express and purify the antigen protein
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  Antigen affinity purification
l  Dot blot confirmation
l  Recombinant vector
l  1mg protein
l  1-2ml pre-bleed
l  2-5mg affinity purified antibody
l  Project datasheet including ELISA and dot blot data
14-16 weeks
Polyclonal package 9
(Phospho-specific polyclonal antibody)
l  Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
l  Synthesize one peptide (12-22aa, purity>85%)
l  Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  Antigen affinity purification
l  2-3mg peptide/EA (non- & phosphor- peptide)
l  1-2ml pre-bleed
l  2-5mg antibody against unphospho-peptide
l  2-5mg phospho-specific antibody
l  Project datasheet including ELISA and dot blot data
13-16 weeks
Polyclonal package 10
(Lys mono-methylated specific polyclonal antibody)
l  Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
l  Synthesize one peptide (12-22aa, purity>85%)
l  Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  Antigen affinity purification
l  2-3mg peptide/EA (non- & mono-methylated peptide)
l  1-2ml pre-bleed
l  2-5mg antibody against unmethylated peptide
l  2-5mg Lys(Me) specific antibody
l  Project datasheet including ELISA and dot blot data
13-16 weeks
Polyclonal package 11  
(Lys dimethylated specific polyclonal antibody)
l  Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
l  Synthesize one peptide (12-22aa, purity>85%)
l  Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  Antigen affinity purification
l  2-3mg peptide/EA (non- & di-methylated peptide)
l  1-2ml pre-bleed
l  2-5mg antibody against unmethylated peptide
l  2-5mg Lys(Me2) specific antibody
l  Project datasheet including ELISA and dot blot data
13-16 weeks
Polyclonal package 12  
(Lys trimethylated specific polyclonal antibody)
l  Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
l  Synthesize one peptide (12-22aa, purity>85%)
l  Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  Antigen affinity purification
l  2-3mg peptide/EA (non- & tri-methylated peptide)
l  1-2ml pre-bleed
l  2-5mg antibody against unmethylated peptide
l  2-5mg Lys(Me3) specific antibody
l  Project datasheet including ELISA and dot blot data
13-16 weeks
Polyclonal package 13 
(Arg methylation-specific polyclonal antibody)
l  Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
l  Synthesize one peptide (12-22aa, purity>85%)
l  Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  Antigen affinity purification
l  2-3mg peptide/EA (non- & methylated peptide)
l  1-2ml pre-bleed
l  2-5mg antibody against unmethylated peptide
l  2-5mg Arg(Me) specific antibody
l  Project datasheet including ELISA and dot blot data
13-16 weeks
Polyclonal package 14   
(Arg di-methylation (Symmetry)-specific polyclonal antibody)
l  Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
l  Synthesize one peptide (12-22aa, purity>85%)
l  Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  Antigen affinity purification
l  2-3mg peptide/EA (non- & dimethylated peptide)
l  1-2ml pre-bleed
l  2-5mg antibody against unmethylated peptide
l  2-5mg Arg(Me2) specific antibody
l  Project datasheet including ELISA and dot blot data
13-16 weeks
Polyclonal package 15 
(Arg di-methylation (Asymmetric)-specific polyclonal antibody)
l  Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
l  Synthesize one peptide (12-22aa, purity>85%)
l  Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  Antigen affinity purification
l  2-3mg peptide/EA (non- & dimethylated peptide)
l  1-2ml pre-bleed
l  2-5mg antibody against unmethylated peptide
l  2-5mg Arg(Me2) specific antibody
l  Project datasheet including ELISA and dot blot data
13-16 weeks
Polyclonal package 16
(Western guaranteed polyclonal antibody)
l  Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
l  Synthesize one peptide (12-22aa, purity>85%)
l  Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  Antigen affinity purification
l  Western blot confirmation
l  2-3mg antigen peptide
l  1-2ml pre-bleed
l  2-5mg affinity purified antibody
l  Project datasheet including ELISA and Western blot data
15-18 weeks
Polyclonal package 17
(Western and IHC guaranteed polyclonal antibody)
l  Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
l  Synthesize one peptide (12-22aa, purity>85%)
l  Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  Antigen affinity purification
l  WB and IHC confirmation
l  2-3mg antigen peptide
l  1-2ml pre-bleed
l  2-5mg affinity purified antibody
l  Project datasheet including ELISA,  Western blot and IHC data
15-18 weeks