Modified Primers…

We offer a broad spectrum of modified primers (oligo modifications) incorporated into an oligonucleotide available as a 5′-, 3- and internal modification. Our modified primers (oligo modifications) are purified by:

  • Reverse Phase Column
  • Reverse Phase HPLC

and available in the following synthesis scales:

  • 0,04 µmol (standard synthesis scale; most popular)
  • 0,2 µmol (medium scale)
  • 1,0 µmol (large scale)

Specification for DNA Reverse Phase Column (RPC) modified primers:

  • Deprotected & purified
  • Recommended for methods like PCR, sequencing and hybridization
  • Delivered as solid
  • Modification allowed (see price list for available modifications purified by RPC)
  • Certificate of Analysis (CoA) follows
  • 100% Quality Controlled (QC) by MassSpec
  • To be kept frozen at -18 C
  • Average in-house turnover time: 3-5 working days
  • Scale & yield
    Product Synthesis scale (µmol) Length OD Est. no. of PCR reactions
    DNA Reverse Phase Column 0.01 (n/a) n/a n/a n/a
    DNA Reverse Phase Column 0.04 8-60 3 300
    DNA Reverse Phase Column 0.20 8-60 12 1,200
    DNA Reverse Phase Column 1.00 n/a n/a n/a
    DNA Reverse Phase Column Plate Oligos 96 0.04 8-60 3 300

Specification for DNA Reverse Phase HPLC (RP-HPLC) modified primers:

  • Deprotected & purified
  • Recommended for methods like PCR, sequencing and hybridization
  • Delivered as solid
  • Modification allowed (see price list for available modifications purified by HPLC)
  • Certificate of Analysis (CoA) follows
  • 100% Quality Controlled (QC) by MassSpec
  • To be kept frozen at -18 C
  • Average in-house turnover time: 3-5 working days
  • Scale & yield
    Product Synthesis scale (µmol) Length OD Est. no. of PCR reactions
    DNA Reverse Phase HPLC 0.01 (n/a) n/a n/a n/a
    DNA Reverse Phase HPLC 0.04 8-80 2 200
    DNA Reverse Phase HPLC 0.20 8-80 4 400
    DNA Reverse Phase HPLC extra long 0.20 81-120 2 200
    DNA Reverse Phase HPLC 1.00 8-80 20 20.000

Specifications for RNA Reverse Phase HPLC (RP-HPLC) modified primers:

  • Deprotected & purified
  • Recommended for methods like PCR, sequencing and hybridization
  • Delivered as solid
  • Modification allowed
  • Certificate of Analysis (CoA) follows
  • 100% Quality Controlled (QC) by MassSpec
  • To be kept frozen at -18 C
  • Average in-house turnover time: 2-3 weeks
  • Scale & yield
    Product Synthesis scale (µmol) Length OD Est. no. of PCR reactions
    RNA Reverse Phase HPLC 0.01 (n/a) n/a n/a n/a
    RNA Reverse Phase HPLC 0.04 15-30 15 1.500

List of available oligo modifications:

Price list for Oligo Modifications:

Please inquire should you have any other Oligo Modification requirements.

>>> Order Modified Primers here