Custom Peptide…

Custom Peptide Purity

Custom peptide are purified with a Reversed Phase HPLC (RP-HPLC). We offer eight different purity grades for our custom peptide: crude, desalted, >75%, >85%, >90% >95%, >98% and >99%.

Modifications

A full range of custom peptide modifications such as simple and complex peptides, short and long peptides, normal and special amino acids, biotinylation, phosphorylation, dye labelling, disulfide bonds, etc. Click here for further information.

Length of residue

Our standard custom peptide is 6-30 amino acid residues in length. Though we do produce <6 and >30 amino acid residues in length according to our the specifications of our customers  (60 amino acid residues in length).

Yields

Our production scale of custom peptide is from milligram to multi-gram quantities.

Content

The percentage of the total peptide present in the product is approx. 65%. In addition to TFA, the rest is water and other salts.

Delivery form

Custom peptides are standard delivered as trifluoroacetate salt, but acetate or chloride salts on request. Custom peptides are delivered lyophilized. Poorly soluble custom peptides are specially marked.

Certificate of Analysis

Certificate of Analysis (CoA) follows all custom peptides, incl. HLPC and MassSpec analyses.

Storage & handling

Custom peptides are to be kept frozen at -18 C.

Delivery time

Delivery of our custom peptides is 3-5 weeks.

Handling & Storage

  • The peptides are supplied lyophilized and should be handled with the following considerations:
  • The peptides should be preserved at a cool dry, evades light place, seal.
  •  Short term storage (2 to 3 months) of lyophilized peptide should be done in a freezer at -20 C. For longer storage, we recommend storing the peptide in a deep freezer at -80 C if available.
  • If peptide samples need to be frequently or periodically taken from the stock, it is recommended to make a series of aliquots from the stock.
  • Peptide sequences containing C, M, or W are prone to air oxidation. It is recommended to purge the air out of the vial and replace it with a blanket of nitrogen or argon.
Solubilizing Peptides
The steps outlined below provides you with a method for determining the best solvent for a synthetic peptide based on its amino acid sequence. It is best to first solubilize a small aliquot of the sample, rather than the entire sample.
Assign a value of -1 to each acidic residue. The acidic residues are Asp (D), Glu (E), and the C-terminal -COOH. Assign a value of +1 to each basic residue. The basic residues are Arg (R), Lys (K), His (H), and the N-terminal -NH2. Calculate the overall charge of the peptide.
If the overall charge of the peptide is a positive value, you have a basic peptide. Initially try to dissolve the peptide in water. If the peptide does not dissolve, try 10% and higher solutions of acetic acid. If the peptide still does not dissolve, add TFA (<50ul) to solubilize the peptide and dilute to 1ml with deionized water.
If the overall charge of the peptide is a negative value, you have an acidic peptide. Initially try to dissolve the peptide in water. If the peptide does not dissolve, add NH4OH (<50ul) and dilute to 1ml with deionized water.
If the overall charge of the peptide is zero, your peptide is considered neutral. Neutral peptides may require the addition of organic solvents, such as acetonitrile, methanol, or isopropanol. The addition of denaturants, such as urea or guanidinium-HCL may also be required.

Price list for Custom Peptides:

Please inquire should you have any other Custom Peptide requirements.

>>> Order Custom Peptide here

Please note: all peptides listed in this website are for research or production use only, not for direct human use.