RNA Oligos

RNA Oligos

TAG Copenhagen offers custom RNA oligo synthesis. Our RNA oligos are synthetic synthesized under the highest quality standards.

Chemically-synthetic siRNA Oligos

  • Custom siRNA Oligo
  • Chemically Modified siRNA Oligo
  • Fluorescent Dye Labeled siRNA Oligo
  • siRNA control
  • siRNA set

Custom siRNA Oligos

Quality control

All oligos undergo vigorous monitoring and stringent quality control. Produced under ISO 9001:2015 quality standard system. Products are quantified by spectrophotometer

Purification

HPLC. siRNA content >97%

Modification

3′ and 5′ end Biotin, FAM and Phosphorate

Length

19-23 bases/each dsRNA

Product style

Single-stranded aliquots. Annealing dsRNA aliquots.

Storage and stability

Although oligonucleotides are stable in solution at 4 C for up to 2 weeks, TAG Copenhagen recommends storage should be at -20 C. Repetitive freeze-thaw cycles should be avoided by storing as aliquots. Storing at concentrations above 20 mikroM is recommended. TAG Copenhagen guarantees its oligonucleotides for 6 months, stored under the above conditions.

Technical Data Sheet

Oligonucleotides are delivered with an oligonucleotide technical data sheet, which includes oligo name, sequence, concentration precise quantity in OD and nmols, Tm, MW, size, extinction coefficient and purification data.

Personal service

Aliquot products according to customer’s request.

Shipment

Shipped by express delivery in 1,5 ml transparent tubes in dried powder.

Custom siRNA Oligo

Cat. #Synthesis scaleSequenceQuantity (OD)PurificationDelivery (days)
TAG0100110 nmol Custom siRNA 19-232HPLC14
TAG0100340 nmol Custom siRNA 19-234HPLC14
TAG01007200 nmol Custom siRNA 19-238HPLC14
TAG010101.000 nmol Custom siRNA 19-2325HPLC14

Chemically Modified siRNA Oligo

The biggest challenge of RNAi technology is the siRNA stability problem. TAG Copenhagen modified siRNA can obtain the best result.

  • Highly effective gene knockdown
  • High stability – in blood serum and culture medium
  • Greatest limit reducing side-effect
  • Longer response time
ConcernStandard siRNAsiRNA
siRNA DegradationUmodified RNA quickly degrades in cell culture and is unlikely to ahve extended longevity in cell and culture and be effective in most in vivo applicationTAG Copenhagen modified siRNA results in greater longevity in cell culture and stability in cell culture and in serum, enhancing the ability for this to be used for in vivo application.
Long-lasting effectShort time, most of all is about one week or so.TAG Copenhagen chemical modified siRNA has much more extension effective time than Standard siRNA.
In vivo ActivityStandard siRNA haas lower stability and not suitable in vivo application.TAG Copenhagen chemical modified siRNA has high stability in most in vivo application.

Chemically Modified siRNA Oligo

Terminal ModificationSynthesis scale (nmol)Quantity (OD)PurificationDelivery (days)
5’ FAM2005HPLC14
5’ Cy32005HPLC14
5’ Biotin2005HPLC14
5’ Amine2005HPLC14
5’ Phosphate2005HPLC14
5’ Thiol2005HPLC14
5’ Cholesterol2005HPLC14
3’ Amine2005HPLC14
Internal ModificationSynthesis scale (nmol)Quantity (OD) Purification Delivery (days)
2’ Fluoro dU 2005HPLC14
2’ Fluoro dC 2005HPLC14
2’ OMe rU 2005HPLC14
2’ OMe rC 2005HPLC14
2’ OMe rG 2005HPLC14
2’ OMe rA 2005HPLC14
Cat. #Internal Modification Synthesis scale (nmol)Quantity (OD)PurificationPurification
TAG020012’-O-Me or 2’-Fluoro102HPLC14
TAG020032’-O-Me or 2’-Fluoro404HPLC14
TAG020072’-O-Me or 2’-Fluoro2008HPLC14
TAG020102’-O-Me or 2’-Fluoro100025HPLC14

Fluorescent Dye Labeled siRNA Oligo

The Fluorescent dye Labeled siRNA oligo can be detected  by Flow Cytometry, fluorescence microscope, laser co-focus microschope and so on, dermined transfection efficacy, optimizing transfection condition.

Cat. #Fluorescent Dye Labeled siRNA OligoSynthesis scale (nmol)Quantity (OD)PurificationDelivery (days)
TAG03001FAM or Cy3102HPLC14
TAG03003FAM or Cy3404HPLC14
TAG03007FAM or Cy32008HPLC14
TAG03010FAM or Cy3100025HPLC14

siRNA control

Cat. #  Negative Control Synthesis scale (nmol) Quantity (OD)Purification Delivery (days)
TAG06001Custom Negative Control101 ODHPLC14
TAG07001Control siRNA101 ODHPLC14
TAG08001Positive Control siRNA101 ODHPLC14

Positive Control

Often used siRNA positive control:

  • LaminA/C
  • GFP22
  • Luciferase GL2
  • MAPK1
  • Beta-Actin
  • Vimentin
  • P53
  • GAPDH
  • Cyclophilin B

siRNA set

We offer six different economical sets for your choice.

Common siRNA Economical Set-A
ProductsQuantityPurification
Target Gene siRNA Oligos3 candidates (1 x 2 OD)HPLC
Negative Control1 ODHPLC
FAM-labeled Negative Control1 ODHPLC
Positive Control siRNA Oligos1 ODHPLC
Common siRNA Beneficial Set-B
Products Quantity Purification
Target Gene siRNA Oligos4 candidates (1 x 4 OD)HPLC
Negative Control1 ODHPLC
FAM-labeled Negative Control1 ODHPLC
Positive Control siRNA Oligos1 ODHPLC
Chemically Modified siRNA Economical Set-C
ProductsQuantityPurification
Target Gene siRNA Oligos3 candidates (1 x 2 OD)HPLC
Negative Control1 ODHPLC
FAM-labeled Negative Control1 ODHPLC
Positive Control siRNA Oligos1 ODHPLC
Common siRNA Beneficial Set-D
ProductsQuantityPurification
Target Gene siRNA Oligos4 candidates (1 x 4 OD)HPLC
Negative Control1 ODHPLC
FAM-labeled Negative Control1 ODHPLC
Positive Control siRNA Oligos1 ODHPLC
Fluorescently-labeled siRNA Economical Set-E
ProductsQuantityPurification
Target Gene siRNA Oligos3 candidates (1 x 2 OD)HPLC
Negative Control1 ODHPLC
FAM-labeled Negative Control1 ODHPLC
Positive Control siRNA Oligos1 ODHPLC
Fluorescently-labeled siRNA Beneficial Set-F
ProductsQuantityPurification
Target Gene siRNA Oligos4 candidates (1 x 4 OD)HPLC
Negative Control1 ODHPLC
FAM-labeled Negative Control1 ODHPLC
Positive Control siRNA Oligos1 ODHPLC

Sets economical prices

Cat. #ProductsQuantityPurificationDelivery (days)
TAG10001Set-A1 setHPLC14
TAG10002Set-B1 setHPLC14
TAG10003Set-C1 setHPLC14
TAG10004Set-D1 setHPLC14
TAG10005Set-E1 setHPLC14
TAG10006Set-F1 setHPLC14

Price list for Custom RNA Oligos

  • The price for Custom RNA Oligos is available online – Click here

Please contact our customer service for a quotation and when ordering custom RNA Oligos.